X-gal staining of canine skin tissues: A technique with multiple possible applications
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چکیده
منابع مشابه
X-gal staining of Drosophila embryos compatible with antibody staining or in situ hybridization.
the slow development. The low background especially applies for detection of digoxigenin-labeled RNA probes. When detecting identical probes labeled with fluorescein, the background is higher, presumably because of nonspecific binding of the fluorescein antibody (the higher background is detected with both BCIP and NBT/BCIP). In cases in which the investigated signals are of different intensity...
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Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique is regularly used, it requires high quality of specific antibodies and there is no good quality of antibody available for certain proteins. Alternativ...
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X-gal staining is a common procedure used in the histochemical monitoring of gene expression by light microscopy. However, this procedure does not permit the direct confocal acquisition of images, thus preventing the identification of labelled cells on the depth (Z) axis of tissue sections and leading sometimes to erroneous conclusions in co-localization and gene expression studies. Here we rep...
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When trypan blue is injected intravenously, the animal appears blue within a few minutes, not only in its integument, but in the internal organs as well. 12 or more hours after the administration of the dye, there are innumerable granules within macrophages and other colloidopexic cells, easily demonstrable by ordinary histological technique. But the animal is blue long before such granules are...
متن کاملGolgi Staining Technique
The Golgi staining technique, also called the black reaction after the stain's color, was developed in the 1870s and 1880s in Italy to make brain cells (neurons) visible under the microscope [3]. Camillo Golgi developed the technique while working with nervous tissue, which required Golgi to examine cell structure under the microscope [3]. Golgi improved upon existing methods of staining, enabl...
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ژورنال
عنوان ژورنال: Journal of Natural Science, Biology and Medicine
سال: 2014
ISSN: 0976-9668
DOI: 10.4103/0976-9668.136147